![[-]](https://www.horuset.com/images/igame/collapse.png "[-]")
29-04-2023, 09:47 PM
Genetic experimentation in the name of House Horuset
Phase one: Genetic splicing of Hirudo Vitosus and Homo Sapiens
Day 1; Session 1:
10:00 PM
Acolyte Occularis
Work began on the project and sample 1 was gathered from the H. Sapiens tissue. Analysis was performed upon sample in order to gauge for any large scale cellular and genetic damage, limited cellular damage and almost no genetic damage was found due to preservation of tissue immediately after retrieval. Genetic material was extracted from the sample using restriction nuclease to cleave the DNA, genetic material was then flash frozen in an appropriate suspension fluid. A number of suitable donor cells were acquired and had their nuclei cleaned of all genetic material, these cells were then also flash frozen in preparation.
Day 2; Session 111:20 AMAcolyte Occularis
Request was submitted to the sith Cautus’ assistant for tissue samples of the Hirudo Vitiosus (K’lor’slug) that he had in his custody, request was granted.
Day 2; Session 26:00 PMAcolyte Occularis
Upon receiving tissue sample from Hirudo Vitiosus two chromosomal elements were removed using nuclease cleaving; [chromosome 23, nc_007134.6 (17999936..18006800)] and [chromosome 2, nc_015868.2 (17798135..17806125)] these are responsible for the generation of Vitiosus’ chitinase and venom. Seven of these pairs were inserted into seven of the donor cells along with the main subject’s DNA using microinjection directly into the empty nuclear envelope. Each of the cells, now labelled subjects 1 through 7, have the Vitiosus chromosomal information inserted at different configurations.
All cells were then inserted into solution closely mimicking the temperature and chemical composition of the human body.
Day 3; Session 11 PMAcolyte Occularis
All subjects were inspected, subjects 1, 3, 4, 6 show promise in acceptance of foreign DNA. Subjects 2, 5 and 7 show possible indications of rejection, and will be monitored in order to assess if disposal will be necessary. In the meantime all samples were placed into artificial wombs with an extremely concentrated aggressive nutrient and catalyst solution. A solution of this concentration would usually cause a rapid growth rate at the consequence of intense mental instability in those grown in it, however all subjects will be lobotomized regardless should they advance in gestation successfully as this is merely a physiological test.
Day 5; Session 111 AMAcolyte Occularis
Monitoring was conducted on all samples, samples 2 and 7 had rejected early in gestation, resulting in unusable embryos. Samples 2 and 7 were disposed of via incineration. Sample 5 still shows signs of irregularity, and the four optimal samples maintain good condition at the Blastocyst level, cellular division is well under way and stem cells have begun cell specialisation.
Sample 1: Optimal / Sample 2: Disposed / Sample 3: Optimal / Sample 4: Optimal / Sample 5: Poor / Sample 6: Optimal / Sample 7: Disposed
Day 5; Session 23 AMAcolyte Occularis
Sample 5 has failed and been incinerated, samples 1, 3, 4 and 6 have shown cell specialisation divergent from typical human genetic makeup, as hoped. Elevated levels of chitinase production cells have been detected in all Blastocysts, and Hirudo Vitiosus’ signature venom has been detected in samples 1 and 4. Samples 1, 3, 4 and 6 have been labelled subjects 1, 2, 3 and 4 from now on.
Sample 1: Chitinase and Venom / Sample 3: Chitinase / Sample 4: Chitinase and Venom / Sample 5: Disposed / Sample 6: Chitinase
Day 8; Session 12 AMAcolyte Occularis
Subjects all appear to be in good health, subject 3 has developed vestigial chitinous formations on what will become it’s arms, perhaps an indication of claws. Subject 1 appears to be growing a venom gland already, subject 2 has chitinous growths on both its head and legs, suggesting that it may grow armour, and subject 4 appears to be developing in line with a normal human child, unfortunately.
Subject 1: Chitinase and Venom / Subject 2: Chitinase / Subject 3: Chitinase and Venom / Subject 4: Chitinase
Day 9; Session 17 PMAcolyte Occularis
It would appear that the early development of venom glands in sample 1 was not ideal. In its vestigial, flawed form the gland appears to have lacked integrity, resulting in the venomous secretions flooding subject 1’s cardiac system and causing mass organ failure. Subject was incinerated after autopsy, which revealed effects identical to recorded deaths to Hirudo Vitiosus venom, as well as small plaques of chitin forming upon various muscles within the subject. In review, these plaques would have certainly limited mobility and thus rendered any further growth of subject 1 a waste of resources regardless of venom. Subjects 2 through 4 remain healthy, although subject 4 still shows few signs of adaption.
Subject 1: Terminated / Subject 2: Chitin plating / Subject 3: Chitin claws and latent venom production genes / Subject 4: Chitinase production
Day 14; Session 17 PMAcolyte Occularis
Subjects have been under surveillance for some time and have required no special attention or intervention, having maintaining a good condition. The latest battery of tests have bought both positive and negative outcomes to the researcher’s attention. Subject 2’s growth of chitinous armour plating has proven much less fortunate than expected, chitin plates on the subject’s legs have grown to cover the knees of the subject without developing joints for the knees, rendering mobility severely impaired. In turn, chitinous growth’s have covered the subjects eyes, rendering it blind. Subject 2 will be terminated.
Subject 3 remains in good condition and the claws grow as hoped. Subject 4 has surprised us, the reason that chitinase production was detected without any notable armour plating appearing is that Subject 4 has abnormally high levels of chitin in its skin. This will likely result in dramatically increased durability of the skin, perhaps that of hardened leather, without inferior manoeuvrability.
Subject 2: Terminated / Subject 3: Chitin claws and latent venom production genes / Subject 4: Armour skin
Day 15; Session 11 PMAcolyte Occularis
In order to investigate Subject 3’s venom production genes which appeared to not have manifested a medical droid was used to carry out laparoscopic exploratory surgery. Parts of the lymphatic system surrounding the basilic vein have been adapted into a primitive, sealed venom production system. A small gland was found in each hand producing a venom consisting largely of C131H229N39O31 (Melittin) and PLA1A (Phospholipase A) along with a few minor compounds, these glands feed into minute tubes within the chitin claws. A test of the venom on a living target is to be planned.
Subject 3: Chitin claws and venom glands/ Subject 4: Armour skin
Day 15; Session 25 PMAcolyte Occularis
Three Rattus Coruscanti were obtained for the experiment and were administered an exact synthesized copy of the venom from subject 3. The first Rattus Coruscanti, henceforth referred to as subject 0-1, was administered the equivalent dose of a single strike from the claws, adjusted to match the size of the vermin. 0-1 almost immediately showed discomfort and soon after outright agony, as well as fatigue, decreased kidney function and decreased liver function. 0-1 appears to be recovering safely and will be kept for observation.
Subject 0-2 was then administered a dose equivalent to two strikes from the subject’s claws, 0-2 quickly gave in to agony and was found to be comatose after writhing in pain approximately five minutes later. 0-2 appears to have survived the ordeal but has sustained significant damage to the kidneys, liver and elements of the cardiovascular system and will be kept for observation.
Finally, Subject 0-3 was administered the equivalent of three claw strikes. The subject appeared to be in great pain and fell from consciousness in the space of a minute, very quickly going into spontaneous failure of the endocrine system and liver. As the subject began the long process of dying from these issues the suffering was cut short by the breakdown of multiple elements of the cardiovascular system resulting in cardiac arrest. The subject was disposed of after autopsy.
Subject 3: Chitin claws and venom glands/ Subject 4: Armour skin
Day 26; Session 12 PMAcolyte Occularis
In accordance with the recommendation of the Head of Science for House Horuset, Cautus, a model was produced wherein the exact genetic alteration of subject 3 will be encoded into the 46th Y chromosome, and subject 4’s will be inserted into the 46th X chromosome. This will ensure that the heightened chance for tumours and rejection observed in other subjects will not effect a possible breeding population, until more advanced cloning equipment and experience on the researcher’s behalf is obtained.
![[Image: UQjWIR6.png]](http://i.imgur.com/UQjWIR6.png)
All Subjects have reached approximate maturity, although the abnormally aggressive solution they were grown in has rendered them mindless and only capable of a short lifespan. Any future subjects will require longer to gestate.
Phase one: Genetic splicing of Hirudo Vitosus and Homo Sapiens
Day 1; Session 1:
10:00 PM
Acolyte Occularis
Work began on the project and sample 1 was gathered from the H. Sapiens tissue. Analysis was performed upon sample in order to gauge for any large scale cellular and genetic damage, limited cellular damage and almost no genetic damage was found due to preservation of tissue immediately after retrieval. Genetic material was extracted from the sample using restriction nuclease to cleave the DNA, genetic material was then flash frozen in an appropriate suspension fluid. A number of suitable donor cells were acquired and had their nuclei cleaned of all genetic material, these cells were then also flash frozen in preparation.
Day 2; Session 111:20 AMAcolyte Occularis
Request was submitted to the sith Cautus’ assistant for tissue samples of the Hirudo Vitiosus (K’lor’slug) that he had in his custody, request was granted.
Day 2; Session 26:00 PMAcolyte Occularis
Upon receiving tissue sample from Hirudo Vitiosus two chromosomal elements were removed using nuclease cleaving; [chromosome 23, nc_007134.6 (17999936..18006800)] and [chromosome 2, nc_015868.2 (17798135..17806125)] these are responsible for the generation of Vitiosus’ chitinase and venom. Seven of these pairs were inserted into seven of the donor cells along with the main subject’s DNA using microinjection directly into the empty nuclear envelope. Each of the cells, now labelled subjects 1 through 7, have the Vitiosus chromosomal information inserted at different configurations.
All cells were then inserted into solution closely mimicking the temperature and chemical composition of the human body.
Day 3; Session 11 PMAcolyte Occularis
All subjects were inspected, subjects 1, 3, 4, 6 show promise in acceptance of foreign DNA. Subjects 2, 5 and 7 show possible indications of rejection, and will be monitored in order to assess if disposal will be necessary. In the meantime all samples were placed into artificial wombs with an extremely concentrated aggressive nutrient and catalyst solution. A solution of this concentration would usually cause a rapid growth rate at the consequence of intense mental instability in those grown in it, however all subjects will be lobotomized regardless should they advance in gestation successfully as this is merely a physiological test.
Day 5; Session 111 AMAcolyte Occularis
Monitoring was conducted on all samples, samples 2 and 7 had rejected early in gestation, resulting in unusable embryos. Samples 2 and 7 were disposed of via incineration. Sample 5 still shows signs of irregularity, and the four optimal samples maintain good condition at the Blastocyst level, cellular division is well under way and stem cells have begun cell specialisation.
Sample 1: Optimal / Sample 2: Disposed / Sample 3: Optimal / Sample 4: Optimal / Sample 5: Poor / Sample 6: Optimal / Sample 7: Disposed
Day 5; Session 23 AMAcolyte Occularis
Sample 5 has failed and been incinerated, samples 1, 3, 4 and 6 have shown cell specialisation divergent from typical human genetic makeup, as hoped. Elevated levels of chitinase production cells have been detected in all Blastocysts, and Hirudo Vitiosus’ signature venom has been detected in samples 1 and 4. Samples 1, 3, 4 and 6 have been labelled subjects 1, 2, 3 and 4 from now on.
Sample 1: Chitinase and Venom / Sample 3: Chitinase / Sample 4: Chitinase and Venom / Sample 5: Disposed / Sample 6: Chitinase
Day 8; Session 12 AMAcolyte Occularis
Subjects all appear to be in good health, subject 3 has developed vestigial chitinous formations on what will become it’s arms, perhaps an indication of claws. Subject 1 appears to be growing a venom gland already, subject 2 has chitinous growths on both its head and legs, suggesting that it may grow armour, and subject 4 appears to be developing in line with a normal human child, unfortunately.
Subject 1: Chitinase and Venom / Subject 2: Chitinase / Subject 3: Chitinase and Venom / Subject 4: Chitinase
Day 9; Session 17 PMAcolyte Occularis
It would appear that the early development of venom glands in sample 1 was not ideal. In its vestigial, flawed form the gland appears to have lacked integrity, resulting in the venomous secretions flooding subject 1’s cardiac system and causing mass organ failure. Subject was incinerated after autopsy, which revealed effects identical to recorded deaths to Hirudo Vitiosus venom, as well as small plaques of chitin forming upon various muscles within the subject. In review, these plaques would have certainly limited mobility and thus rendered any further growth of subject 1 a waste of resources regardless of venom. Subjects 2 through 4 remain healthy, although subject 4 still shows few signs of adaption.
Subject 1: Terminated / Subject 2: Chitin plating / Subject 3: Chitin claws and latent venom production genes / Subject 4: Chitinase production
Day 14; Session 17 PMAcolyte Occularis
Subjects have been under surveillance for some time and have required no special attention or intervention, having maintaining a good condition. The latest battery of tests have bought both positive and negative outcomes to the researcher’s attention. Subject 2’s growth of chitinous armour plating has proven much less fortunate than expected, chitin plates on the subject’s legs have grown to cover the knees of the subject without developing joints for the knees, rendering mobility severely impaired. In turn, chitinous growth’s have covered the subjects eyes, rendering it blind. Subject 2 will be terminated.
Subject 3 remains in good condition and the claws grow as hoped. Subject 4 has surprised us, the reason that chitinase production was detected without any notable armour plating appearing is that Subject 4 has abnormally high levels of chitin in its skin. This will likely result in dramatically increased durability of the skin, perhaps that of hardened leather, without inferior manoeuvrability.
Subject 2: Terminated / Subject 3: Chitin claws and latent venom production genes / Subject 4: Armour skin
Day 15; Session 11 PMAcolyte Occularis
In order to investigate Subject 3’s venom production genes which appeared to not have manifested a medical droid was used to carry out laparoscopic exploratory surgery. Parts of the lymphatic system surrounding the basilic vein have been adapted into a primitive, sealed venom production system. A small gland was found in each hand producing a venom consisting largely of C131H229N39O31 (Melittin) and PLA1A (Phospholipase A) along with a few minor compounds, these glands feed into minute tubes within the chitin claws. A test of the venom on a living target is to be planned.
Subject 3: Chitin claws and venom glands/ Subject 4: Armour skin
Day 15; Session 25 PMAcolyte Occularis
Three Rattus Coruscanti were obtained for the experiment and were administered an exact synthesized copy of the venom from subject 3. The first Rattus Coruscanti, henceforth referred to as subject 0-1, was administered the equivalent dose of a single strike from the claws, adjusted to match the size of the vermin. 0-1 almost immediately showed discomfort and soon after outright agony, as well as fatigue, decreased kidney function and decreased liver function. 0-1 appears to be recovering safely and will be kept for observation.
Subject 0-2 was then administered a dose equivalent to two strikes from the subject’s claws, 0-2 quickly gave in to agony and was found to be comatose after writhing in pain approximately five minutes later. 0-2 appears to have survived the ordeal but has sustained significant damage to the kidneys, liver and elements of the cardiovascular system and will be kept for observation.
Finally, Subject 0-3 was administered the equivalent of three claw strikes. The subject appeared to be in great pain and fell from consciousness in the space of a minute, very quickly going into spontaneous failure of the endocrine system and liver. As the subject began the long process of dying from these issues the suffering was cut short by the breakdown of multiple elements of the cardiovascular system resulting in cardiac arrest. The subject was disposed of after autopsy.
Subject 3: Chitin claws and venom glands/ Subject 4: Armour skin
Day 26; Session 12 PMAcolyte Occularis
In accordance with the recommendation of the Head of Science for House Horuset, Cautus, a model was produced wherein the exact genetic alteration of subject 3 will be encoded into the 46th Y chromosome, and subject 4’s will be inserted into the 46th X chromosome. This will ensure that the heightened chance for tumours and rejection observed in other subjects will not effect a possible breeding population, until more advanced cloning equipment and experience on the researcher’s behalf is obtained.
All Subjects have reached approximate maturity, although the abnormally aggressive solution they were grown in has rendered them mindless and only capable of a short lifespan. Any future subjects will require longer to gestate.
Discord:
lord_saltaeon
